Risk factors of pelvic floor muscle strength in south Chinese women: a retrospective study

Objectives To evaluate pelvic floor muscle strength using surface electromyography and risk factors for pelvic floor muscle strength in the early postpartum period. Methods This retrospective study included 21,302 participants who visited Fujian Maternity and Child Health Hospital from September 2019 to February 2022. All participants were assessed by medical professionals for general information and surface electromyography. Results Univariate analysis indicated that age was inversely related to tonic and endurance contractions. In contrast, all the other variables, including education level, body mass index, neonatal weight, and number of fetuses, had a positive impact on rapid, tonic, and endurance contractions. Likewise, parity was also positively associated with rapid contractions. In addition, compared with vaginal delivery, cesarean section delivery had a protective effect on the amplitude of the three types of contractions. Stepwise regression analysis showed that both age and neonatal weight had a negative linear relationship with the amplitude of rapid, tonic and endurance contractions. In contrast, the amplitude of rapid, tonic and endurance contractions significantly increased as body mass index, parity (≤ 3), education level and gestational weight gain (endurance contractions only) increased. Participants with cesarean section delivery showed positive effects on rapid, tonic, and endurance contractions compared to participants with vaginal delivery. Conclusions We found that age, neonatal weight, vaginal delivery, episiotomy, and forceps delivery were risk factors for pelvic floor muscle strength; in contrast, body mass index, parity (≤ 3) and gestational weight gain had a positive relationship with pelvic floor muscle strength.


Introduction
The pelvic floor is composed of three layers of muscles combined with ligaments and fascia that act as a sling to support the bladder, reproductive organs, and rectum [1,2]. The pelvic floor muscles (PFMs) function to regulate the storage and evacuation of urine and stool by coordinated contraction and relaxation. In normal people, for the purpose of preventing urinary incontinence (UI), the PFMs needs to be flexible enough to contract, providing additional external support for the urethra to cope with sudden increased intra-abdominal pressure, such as the pressure induced by coughing [3]. Similarly, the contraction of the PFMs also plays an important role in anal continence function [4]. Both pregnancy and subsequent vaginal delivery may lead to levator plate relaxation and thus increase the risk of developing pelvic floor dysfunctions (PFDs), especially UI [5][6][7][8]. The prevalence Open Access *Correspondence: 3064513770@qq.com of UI in adult women was approximately 22.1% in China, 16.7% in Japan, 53% in the United States, 35.3% in Australia, and 36.3% in Saudi countries, and the prevalence of fecal incontinence (FI) was 8.39% in America, 3.6% in the United Kingdom, and 4.2% in Italy based on large population-studies [9][10][11][12][13][14][15]. PFDs are serious problems that can decrease participation in sports and social functions, and that have an indisputable impact on quality of life [16,17]. With increasing age, the volume of PFMs decreases, the strength of PFMs weakens, and the incidence of PFDs increases [18,19]. In contrast, stronger PFM strength has a great protective effect on the pelvic floor and reduces the occurrence of PFDs [20,21].
Therefore, it is important to assess PFM strength and determine the factors that may affect it. The aim of this study was to evaluate PFM strength using surface electromyography (EMG) and risk factors for PFM strength in the early postpartum period.

Participants
This retrospective study included 21,302 participants who visited Fujian Maternity and Child Health Hospital from September 2019 to February 2022. All participants were assessed by medical professionals for general information and surface EMG. The inclusion criteria were as follows: participants who were 40 days to 6 months postpartum and who could tolerate a gynecological examination [22]. The exclusion criteria were: participants with gynecologic bleeding, those suspected of being pregnant, and those who had undergone urogynecological and gynecological surgeries. This study was approved by the Ethics Committee of Fujian Maternity and Child Health Hospital (No. 2022KYLLR03046).

Assessment of pelvic floor surface EMG
In China, the assessment of EMG has been routinely used to evaluate pelvic floor conditions for years [23]. A human biostimulation feedback instrument (MLD B2T, Medlander, Najing, Jiangsu, China) was used to evaluate the EMG of the participants, including pretest resting, rapid contractions, tonic contractions, endurance contractions, and posttest resting, following the Glazer protocols [24]. The participants who underwent the test were placed in the supine lithotomy position, and then a vaginal probe was placed into the vagina. Electrode configurations were positioned on abdominal muscles to monitor unwanted muscle activation. The evaluator instructed them to perform vaginal contractions, guided by words such as "Please relax your abdomen and hips", "Please contract and relax your vagina or anus quickly" and "Please contract your vagina or anus and holding". Then, the automated protocol software instructed the participants with text hints on a screen and voice prompts. In addition, our staff also supervised participants to avoid false contractions. There was a 30-s study period before the test to ensure that the participants had mastered the test correctly.

Statistical analysis
All statistical analyses were performed using SPSS software version 26.0. Univariable analysis for categorical and continuous parameters was performed with chi square tests and t tests, respectively. The greater the absolute value of the standardized regression coefficient (β), the greater the influence of the corresponding independent variable on the dependent variable. Stepwise regression analysis was used to assess the relationship between the independent and dependent variables. For all tests, a two-tailed p value < 0.05 was considered statistically significant.

Discussion
Assessment of the PFMs is the basis for the prevention of PFDs. Pelvic floor surface EMG is a noninvasive technique that collects muscle motor potentials through surface electrodes, and is considered an effective method to assess the strength of the PFMs [25][26][27]. Previous studies have reported the association of EMG with UI and it is reliable and consistently predictive of clinical status variables [26,28]. Surface EMG is widely used in China for the evaluation of PFM function because of its easy accessibility and cost-effectiveness and it has been considered effective to assess the function of the PFMs according to Branch of Women's Health Care, Chinese Preventive Medicine Association [22]. Therefore, our study may contribute to predicting changes in pelvic floor muscle strength as well as its influencing factors to prevent pelvic floor muscle relaxation in the early stage.
Some sociodemographic characteristics may have an effect on PFM strength. Some studies have reported that aging may lead to a decrease in mechanical strength and predispose an individual to prolapse, UI and sexual dysfunction [29][30][31][32]. Likewise, in our study, the PFM rapid, tonic, and endurance contraction amplitudes all decreased when age increased.
BMI is also closely associated with PFDs. It has been reported that high BMI is a risk factor for PFDs, but it has also been reported that low BMI can also lead to pelvic organ prolapse (POP) [33,34]. Univariate analysis and linear regression found that BMI was positively correlated with PFM strength in this study. In addition, some studies have also reported that GWG increased the subsequent risk of PFDs [35,36]. In this paper, GWG contributed to the amplitude of endurance contractions after delivery. Both the increased BMI and GWG might result in increased intra-abdominal pressure [37]. As a result, the strength of the PFMs increased to sustain the increasing intra-abdominal pressure and visceral weight, similar to the correlation between BMI and muscle strength, and the changes might continue into the postpartum period [38][39][40]. The literature on the association between parity and the risk of PFDs indicates that multiparas are more likely to develop PFDs [41][42][43]. Unlike these outcomes, we found that PFM rapid, tonic, and endurance contraction amplitudes in secundiparas and tertiparas were higher than those in primiparas. This was an interesting outcome and might be an inspiration for us to rethink the effect of parity on PFM strength. Some ultrasoundbased studies have found that injury to and structural deformation of the pelvic floor are independent of parity, suggesting that parity does not affect the pelvic floor as we believe [44,45]. In addition, another study showed that the risk of levator avulsions, symptoms of POP, and clinical findings of POP were the same between primiparas and secundiparas, yet the occurrence of symptoms of POP increased for participants with three or more deliveries when compared to participants with one delivery [46]. Additionally, since sex education was not widespread in China teenagers, multiparas were more likely to receive sex education and Kegel training than primiparas, thus improving PFM strength [47]. Unfortunately, we did not collect information on whether they had received Kegel training.
Some studies have shown a significant relationship between educational level and PFM strength [42,48]. Likewise, in the present study, PFM rapid, tonic, and endurance contraction amplitudes increased as the educational level increased. This result suggests that education increases women's awareness about PFM strength.
As NW increases, the possibility of PFDs also increases. Previous studies have shown that excessive NW might harm PFM strength and was an independent risk factor for PFDs [49,50]. Stepwise regression analysis showed that NW rather than the NOF, had a negative effect on PFM strength, including rapid, tonic, and endurance contractions, which was contrary to the results of univariate analysis. Women who had a baby that weight more than 4 kg or had twins or triplets were more likely to choose CS delivery, which has been confirmed to be a protective factor for PFM strength [51,52]. A total of 34.1% participants with baby < 4 kg chose CS delivery, 66.8% of the participants with baby ≥ 4 kg chose CS delivery, 34.8% of the participants with a single baby chose CS delivery, and 91.6% of  [55,56]. Similarly, we found that the PFM rapid, tonic, and endurance contraction amplitudes in women with NIVD, EP, and FD were all lower than those in CS. Women with EP and FD showed a negative effect on PFM contraction capacity compared with women with NIVD.

Conclusion
In this study, we found that age, NW, NIVD, EP, and FD were risk factors for PFM strength. Although BMI, parity (≤ 3) and GWG had a positive relationship with PFM strength, this is likely due to body's adaptation ability and self-repair ability, rather than the benefits of weight gain or parity.

Limitations
Only female participants were included; the number of multiparas (≥ 3) was too small to observe the changes in PFM strength when parity continued to grow, the assessment of surface EMG alone cannot reflect the overall function of the pelvic floor, and we lacked assessments of pelvic floor associated scales to assess the participants' clinical symptoms, which made it difficult to relate our results to the clinic.

Funding
This was not a funded project.

Availability of data and materials
The datasets analysed during the current study are not publicly available but are available from the corresponding author on reasonable request. Table 3 Stepwise multiple linear regression analysis for the effect of independent variables on rapid, tonic, and endurance contraction BMI body mass index, GWG gestational weight gain, NW neonatal weight, NOF number of fetus, DM delivery mode, CS cesarean section, NIVD non-instrumental vaginal delivery, EP episiotomy, FD forceps delivery, B unstandardized beta, β standardized beta, t t test statistic * p < .05 ** p < .01 *** p < .001